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Test ID LPS Lipase, Serum

Reporting Name

Lipase, S

Useful For

Investigating pancreatic disorders, usually pancreatitis

Specimen Type


Specimen Required

Patient Preparation: Patients should be fasting before the specimen is drawn.


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tube must be centrifuged within 2 hours of draw time.

2. Red-top tube must be centrifuged and aliquoted within 2 hours of draw time.

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  90 days

Reference Values

≥16 years: 10-73 U/L

Reference values have not been established for patients who are <16 years of age.

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
LPS Lipase, S 3040-3


Result ID Test Result Name Result LOINC Value
LPS Lipase, S 3040-3

Clinical Information

Lipases are enzymes that hydrolyze glycerol esters of long-chain fatty acids and produce fatty acids and 2-acylglycerol. Bile salts and a cofactor, colipase, are required for full catalytic activity and greatest specificity. The pancreas is the primary source of serum lipase. Both lipase and colipase are synthesized in the pancreatic acinar cells and secreted by the pancreas in roughly equimolar amounts. Lipase is filtered and reabsorbed by the kidneys. Pancreatic injury results in increased serum lipase levels.


In pancreatitis, lipase becomes elevated at about the same time as amylase (4-8 hours). But lipase may rise to a greater extent and remain elevated much longer (7-10 days) than amylase.


Elevations 2 to 50 times the upper reference have been reported. The increase in serum lipase is not necessarily proportional to the severity of the attack. Normalization is not necessarily a sign of resolution.


In acute pancreatitis, normoamylasemia may occur in up to 20% of such patients. Likewise, the existence of hyperlipemia may cause a spurious normoamylasemia. For these reasons, it is suggested that the 2 assays complement and not exclude each other, and that both enzymes should be assayed.

Clinical Reference

1. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis and ER Ashwood. Philadelphia, WB Saunders Company, 2001

2. Swaroop VS, Chari ST, Clain JE: Acute pancreatitis. JAMA 2004;291:2865-2868

Analytic Time

Same day/1 day

Method Name

Colorimetric Rate Reaction


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (