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Test ID RESLR Respiratory Pathogen Panel, PCR, Varies

Ordering Guidance

This assay is not predicted to detect SARS-coronavirus (CoV), MERS-CoV, or the virus (SARS-CoV-2) causing coronavirus disease-2019 (COVID-19).


It is not recommended that the following tests be concomitantly ordered if this test is ordered:

-FLUMS / Influenza Virus Type A and Type B, and Respiratory Syncytial Virus (RSV), Molecular Detection, PCR, Varies

-LADV / Adenovirus, Molecular Detection, PCR, Varies

-LENT / Enterovirus, Molecular Detection, PCR, Varies

-BPRP / Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies

-MPRP/ Mycoplasmoides pneumoniae, Molecular Detection, PCR, Varies


This test is appropriate for bronchoalveolar lavage or bronchial washings only. For nasopharyngeal swab specimens, order RESPM / Respiratory Pathogen Panel, PCR, Nasopharyngeal.

Shipping Instructions

Specimens that cannot be shipped refrigerated within 3 days (72 hours) should be frozen prior to shipment. Specimens received older than 72 hours (refrigerated) or older than 30 days (frozen) will be canceled.

Specimen Required

Specimen Type: Fluid

Sources: Bronchoalveolar lavage (BAL) or bronchial washings

Container/Tube: Sterile container

Specimen Volume: 1 mL

Useful For

Rapid detection of respiratory infections caused by the following:


-Coronavirus (serotypes HKU1, NL63, 229E, OC43)

-Human metapneumovirus

-Human rhinovirus/enterovirus

-Influenza A (H1, H1-2009, H3)

-Influenza B

-Parainfluenza virus (serotypes 1-4)

-Respiratory syncytial virus (RSV)

-Bordetella pertussis

-Chlamydophila pneumoniae

-Mycoplasmoides (Mycoplasma) pneumoniae


This test is not recommended as a test of cure.

Method Name

Multiplex Polymerase Chain Reaction (PCR)

Reporting Name

Respiratory Pathogen Panel PCR Misc

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 72 hours
  Frozen  30 days

Clinical Information

Respiratory infections are common and generally self-limited in healthy, immunocompetent hosts. Viruses account for a significant percentage of respiratory diseases, but bacteria are also associated with respiratory infections, including pneumonia. Although respiratory illnesses are frequently mild, viruses and bacteria may cause significant morbidity and mortality in immunocompromised hosts (eg, transplant recipients, patients with underlying malignancy); however, there is potential for prolonged shedding of microorganisms or nucleic acids in immunocompromised patients without their necessarily causing clinical disease; laboratory results should be interpreted in the context of clinical findings. Influenza viruses (type A and type B) and respiratory syncytial virus (RSV) are 2 common causes of viral respiratory illness, with peak incidence in the winter and spring months in the Northern hemisphere. Both viruses can cause a clinically indistinguishable syndrome, characterized by fever, cough, headache, and general malaise. RSV is a leading cause of respiratory illness in young children. Early diagnosis of influenza and RSV is important so that 1) necessary infection control precautions can be taken if the patient is hospitalized, and 2) antiviral therapy can be considered if the patient is hospitalized or considered at high-risk for severe disease.(1) Human metapneumovirus is a relative of RSV, and is also a cause of respiratory illness in both children and adults.


Human rhinovirus and coronavirus (serotypes HKU1, NL63, 229E, OC43) are the causative agents of the common cold, with symptoms including runny nose, sore throat, and malaise. Infections with rhinovirus and coronaviruses are common due to the large number of serotypes of these viruses. The vast majority of infections are mild and self-limiting; however, immunocompromised hosts may suffer more severe illness, including lower respiratory tract disease.


Parainfluenza viruses are a common cause of mild, self-limiting viral infections, especially in young children. Infections are most common in the spring, summer, and fall months, with symptoms including fever, runny nose, and cough; however, parainfluenza may also cause more severe lower respiratory disease, such as croup or pneumonia particularly in older adults or immunocompromised patients.


Adenoviruses may infect a range of organ systems, with sequelae ranging from cold-like symptoms (sore throat), to pneumonia, conjunctivitis (pink eye), or diarrhea. Adenoviruses generally cause mild, self-limited infections but may cause severe disease in immunosuppressed patients.


Respiratory infections may also be caused by bacterial pathogens, including Bordetella pertussis, Chlamydophila pneumoniae, and Mycoplasmoides (Mycoplasma) pneumoniae. Bordetella pertussis is the causative agent of pertussis, or whooping cough, a disease characterized by persistent cough that may be associated with an inspiratory whoop and post-tussive vomiting. Mycoplasmoides (Mycoplasma) pneumoniae is a cause of upper respiratory infection, pharyngitis, tracheobronchitis, and pneumonia. Chlamydophila pneumoniae is a rare cause of pneumonia.

Reference Values

Negative (for all targets)


Results of the panel are intended to aid in the diagnosis of illness and are meant to be used in conjunction with other clinical and epidemiological findings.


A negative result should not rule-out infection in patients with a high pretest probability for a respiratory infection. The assay does not test for all potential infectious agents of respiratory disease. Samples collected too early or too late in the clinical course may not yield the organism causing disease. Negative results should be considered in the context of a patient's clinical course and treatment history, if applicable.


Positive results do not distinguish between a viable/replicating organism and the presence of a nonviable organism or nucleic acid, nor do they exclude the potential for coinfection by organisms not contained within the panel. Nucleic acid may persist in some patients for days to weeks, even following appropriate therapy. Detection of 1 or more organisms included in this test suggests that the virus/bacterium is present in the clinical sample; however, the test does not distinguish between organisms that are causing disease and those that are present but not associated with a clinical illness. Coinfections (eg, detection of multiple viruses or bacteria or viruses and bacteria) may be observed with this test. In these situations, the clinical history and presentation should be reviewed thoroughly to determine the clinical significance of multiple pathogens in the same specimen.

Clinical Reference

1. Lee N, Lui GC, Wong KT, et al: High morbidity and mortality in adults hospitalized for respiratory syncytial virus infections. Clin Infect Dis. 2013:57(8):1069-1077

2. Miliander C, Espy M, Binnicker MJ: Evaluation of the BioFire FilmArray for the detection of respiratory viruses in clinical samples. Clinical Virology Symposium Annual Meeting, Daytona, Florida, April 2013

3. Ruggiero P, McMillen T, Tang YW, Babady NE: Evaluation of the BioFire FilmArray Respiratory Panel and the GenMark eSensor Respiratory Viral Panel on Lower Respiratory Tract Specimens. J Clin Microbiol. 2014:52(1):288-290

4. Ramanan P, Bryson AL, Binnicker MJ, Pritt BS, Patel R: Syndromic panel-based testing in clinical microbiology. Clin Microbiol Rev. 2017 Nov 15;31(1):e00024-17. doi: 10.1128/CMR.00024-17

Day(s) Performed

Monday through Sunday

Report Available

1 to 2 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information





LOINC Code Information

Test ID Test Order Name Order LOINC Value
RESLR Respiratory Pathogen Panel PCR Misc 92958-8


Result ID Test Result Name Result LOINC Value
RESRC Specimen Source 31208-2
607861 Adenovirus 92987-7
607862 Coronavirus 229E 94479-3
607863 Coronavirus HKU1 94480-1
607864 Coronavirus NL63 94481-9
607865 Coronavirus OC43 94482-7
607866 Human Rhinovirus/ Enterovirus 92956-2
607867 Human Metapneumovirus 92978-6
607868 Influenza A 92977-8
607869 Influenza B 92976-0
607870 Parainfluenza Virus 1 94483-5
607871 Parainfluenza Virus 2 94484-3
607872 Parainfluenza Virus 3 94485-0
607873 Parainfluenza Virus 4 94486-8
607874 Respiratory Syncytial Virus 92957-0
607875 Bordetella pertussis 94478-5
607876 Chlamydophila pneumoniae 92986-9
607877 Mycoplasmoides (Mycoplasma) pneumoniae 92964-6
601600 Interpretation 59464-8


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.