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Test ID SMA Smooth Muscle Antibodies, Serum

Reporting Name

Anti-Smooth Muscle Ab

Useful For

Evaluating patients with chronic liver disease in whom the diagnosis of chronic active autoimmune hepatitis is suspected

Specimen Type


Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.8 mL

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values


If positive, results are titered.

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 11 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


86256-Titer (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SMA Anti-Smooth Muscle Ab 26971-2


Result ID Test Result Name Result LOINC Value
6284 Anti-Smooth Muscle Ab 26971-2

Clinical Information

Sera from patients with autoimmune chronic active hepatitis contain antibodies to smooth muscle antigens that are detectable by indirect immunofluorescence on substrates that contain smooth muscle. The antibodies are predominantly of the IgG isotype.


Other diseases in this differential diagnosis group include primary biliary cirrhosis, chronic viral hepatitis, and alcoholic chronic hepatitis.


Antibody titers in the range of 80 to 320 occur commonly in patients with active chronic hepatitis; lower titers (usually <80) may occur in the other conditions mentioned earlier.

Clinical Reference

Czaja AJ, Homburger HA: Autoantibodies in liver disease. Gastroenterology 2001;120:239-249

Analytic Time

2 days

Method Name

Indirect Immunofluorescence


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Gastroenterology and Hepatology Client Test Request (T728)