Home
HelpTest ID ANIDE Organism Referred for Identification, Anaerobic Bacteria
Infectious
Reporting Name
Organism Ref for ID, Anaerobic BactUseful For
Identifying anaerobic bacteria involved in human infections
Specimen Type
VariesOrdering Guidance
If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, Minimal Inhibitory Concentration, Varies also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes
Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds
Supplies:
-Anaerobic Transport Tube (T588)
-Infectious Container, Large (T146)
Container/Tube:
Preferred: Anaerobic transport tube
Acceptable: Thioglycollate broth or any other suitable anaerobic transport system
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reference Values
Identification of organism
Day(s) Performed
Monday through Sunday
CPT Code Information
87076-Organism ref for ID, anaerobic bact
87076-Id MALDI-TOF mass spec anaerobe (if appropriate)
87076-Anaerobe Ident (if appropriate)
87153-Anaerobe ident by sequencing (if appropriate)
87077-Identification commercial kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria identification (if appropriate)
87077-Additional identification procedure (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87147 x 3-Serologic agglut method 1 ident (if appropriate)
87147-Serologic agglut method 2 ident (if appropriate)
87147 x 4-Serologic agglut method 3 ident (if appropriate)
87147 x 2-6-Serologic Agglut Method 4 Ident (if appropriate)
87153-Aerobe ident by sequencing (if appropriate)
87798-Identification by PCR (if appropriate)
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.
Clinical Information
Anaerobic bacteria are the greatest component of the human body's normal bacterial microbiota colonizing the skin, oral cavity, and genitourinary and lower gastrointestinal tracts. Their presence is important in promoting vitamin and other nutrient absorption and in preventing infection with disease-causing bacteria.
Anaerobes generally are of low pathogenicity but may possess virulence factors, such as endotoxin or polysaccharide capsules, or produce extracellular toxins. Disease occurs when a large inoculum develops in an area lacking oxygen or with a poor blood supply.
Typical anaerobic infections include peritonitis, abdominal or pelvic abscesses, endometritis, pelvic inflammatory disease, aspiration pneumonia, empyema, lung abscesses, sinusitis, brain abscesses, gas gangrene, and other soft tissue infections. Many Bacteroides produce beta-lactamase and are resistant to penicillins and cephalosporins. Imipenem, metronidazole, and clindamycin are effective agents, although resistance to clindamycin is increasing.
Interpretation
Isolation of anaerobes in significant numbers from well-collected specimens from blood, other normally sterile body fluids, or closed collections of purulent fluid indicates infection with the identified organism.
Cautions
No significant cautionary statements
Clinical Reference
1. Jousimies-Somer HR, Summanen P, Citron DM, et al: Wadsworth Anaerobic Bacteriology Manual. 6th ed. Star Publishing Co; 2002
2. Baron EJ: Approaches to identification of anaerobic bacteria. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:905-908
3. Hall GS: Anaerobic bacteriology. In: Garcia LS, ed. Clinical Microbiology Procedures Handbook. Vol 1. 3rd ed. ASM Press; 2010:section 4
4. Song Y, Finegold SM: Peptostreptococcus, Finegoldia, Anaerococcus, Peptoniphilus, Veillonella, and other anaerobic cocci. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:909-919
5. Hall V, Copsey SD: Propionibacterium, Lactobacillus, Actinomyces, and other non-spore-forming anaerobic gram-positive rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:920-939
6. Stevens DL, Bryant AE, Carroll K: Clostridium. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:940-966
7. Kononen E, Conrads G, Nagy E: Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic gram-negative rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:967-993
Method Description
Appropriately transported organisms are inoculated onto blood agar plates and into thioglycollate broth tubes. After 48 hours of incubation at 35° C in an anaerobic atmosphere, colonies are identified using one or a combination of the following techniques: Gram stain, use of various differential media, aero tolerance testing, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, or 16S ribosomal RNA gene sequencing.(Procop, GW, Church DL, Hall GS, et al: The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott, Williams and Wilkins; 2017:chap 16)
Report Available
8 to 14 daysReject Due To
| Agar plate Received frozen |
Reject |
NY State Approved
YesMethod Name
Dependent on organism submitted, 1 or more of the following methods will be used: Media, Aero Tolerance Testing, Conventional Biochemical Tests, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, or 16S RNA Gene Sequencing
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.