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Test ID ANIDE Organism Referred for Identification, Anaerobic Bacteria


Reporting Name

Organism Ref for ID, Anaerobic Bact

Useful For

Identifying anaerobic bacteria involved in human infections

Specimen Type


Ordering Guidance

If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, Minimal Inhibitory Concentration, Varies also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.

Shipping Instructions

1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.

Necessary Information

1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.

Specimen Required

Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes

Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds


-Anaerobic Transport Tube (T588)

-Infectious Container, Large (T146)


Preferred: Anaerobic transport tube

Acceptable: Thioglycollate broth or any other suitable anaerobic transport system

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)

Reference Values

Identification of organism

Day(s) Performed

Monday through Sunday

CPT Code Information

87076-Organism ref for ID, anaerobic bact

87076-Id MALDI-TOF mass spec anaerobe (if appropriate)

87076-Anaerobe Ident (if appropriate)

87153-Anaerobe ident by sequencing (if appropriate)

87077-Identification commercial kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria identification (if appropriate)

87077-Additional identification procedure (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87147 x 3-Serologic agglut method 1 ident (if appropriate)

87147-Serologic agglut method 2 ident (if appropriate)

87147 x 4-Serologic agglut method 3 ident (if appropriate)

87147 x 2-6-Serologic Agglut Method 4 Ident (if appropriate)

87153-Aerobe ident by sequencing (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5


Result ID Test Result Name Result LOINC Value
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALA Id MALDI-TOF Mass Spec Anaerobe No, (Bill Only) No
ANAID Anaerobe Ident No, (Bill Only) No
ISAN Anaerobe Ident by Sequencing No, (Bill Only) No
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.

Clinical Information

Anaerobic bacteria are the greatest component of the human body's normal bacterial microbiota colonizing the skin, oral cavity, and genitourinary and lower gastrointestinal tracts. Their presence is important in promoting vitamin and other nutrient absorption and in preventing infection with disease-causing bacteria.


Anaerobes generally are of low pathogenicity but may possess virulence factors, such as endotoxin or polysaccharide capsules, or produce extracellular toxins. Disease occurs when a large inoculum develops in an area lacking oxygen or with a poor blood supply.


Typical anaerobic infections include peritonitis, abdominal or pelvic abscesses, endometritis, pelvic inflammatory disease, aspiration pneumonia, empyema, lung abscesses, sinusitis, brain abscesses, gas gangrene, and other soft tissue infections. Many Bacteroides produce beta-lactamase and are resistant to penicillins and cephalosporins. Imipenem, metronidazole, and clindamycin are effective agents, although resistance to clindamycin is increasing.


Isolation of anaerobes in significant numbers from well-collected specimens from blood, other normally sterile body fluids, or closed collections of purulent fluid indicates infection with the identified organism.


No significant cautionary statements

Clinical Reference

1. Jousimies-Somer HR, Summanen P, Citron DM, et al: Wadsworth Anaerobic Bacteriology Manual. 6th ed. Star Publishing Co; 2002

2. Baron EJ: Approaches to identification of anaerobic bacteria. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:905-908

3. Hall GS: Anaerobic bacteriology. In: Garcia LS, ed. Clinical Microbiology Procedures Handbook. Vol 1. 3rd ed. ASM Press; 2010:section 4

4. Song Y, Finegold SM: PeptostreptococcusFinegoldiaAnaerococcusPeptoniphilusVeillonella, and other anaerobic cocci. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:909-919

5. Hall V, Copsey SD: Propionibacterium, Lactobacillus, Actinomyces, and other non-spore-forming anaerobic gram-positive rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:920-939

6. Stevens DL, Bryant AE, Carroll K: Clostridium. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:940-966

7. Kononen E, Conrads G, Nagy E: Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic gram-negative rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:967-993

Method Description

Appropriately transported organisms are inoculated onto blood agar plates and into thioglycollate broth tubes. After 48 hours of incubation at 35° C in an anaerobic atmosphere, colonies are identified using one or a combination of the following techniques: Gram stain, use of various differential media, aero tolerance testing, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, or 16S ribosomal RNA gene sequencing.(Procop, GW, Church DL, Hall GS, et al: The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott, Williams and Wilkins; 2017:chap 16)

Report Available

8 to 14 days

Reject Due To

Agar plate Reject

Method Name

Dependent on organism submitted, 1 or more of the following methods will be used: Media, Aero Tolerance Testing, Conventional Biochemical Tests, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, or 16S RNA Gene Sequencing


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.