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Test ID BFCC Cell Count and Differential, Body Fluid


Ordering Guidance


For spinal fluid, order CSFCC / Cell Count and Differential, Spinal Fluid.

 

For bronchoalveolar lavage, order LAV / Cell Count and Differential, Bronchoalveolar Lavage.



Shipping Instructions


Specimen must arrive within 24 hours of collection.



Necessary Information


Indicate specimen source



Specimen Required


For Local Accounts Only

Sources: Synovial, pleural, peritoneal, pericardial fluid

Container/Tube:

Preferred: Body fluid container

Acceptable: Lavender top (EDTA) or green top (heparin)

Specimen Volume: 1 mL


Useful For

Aiding in the diagnosis of joint disease, systemic disease, inflammation, malignancy, infection, and trauma

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
LCMS Leukemia/Lymphoma, Phenotype Yes No
CYTNG Cytology Non-GYN Yes No

Testing Algorithm

When abnormal cytologic features are present, a miscellaneous cytology test may be added and performed at an additional charge. Fee codes for that test vary depending on review process.

Method Name

Automated or Manual Cell Count/Cytocentrifugation followed by Manual Differential and Morphology Review

Reporting Name

Cell Count and Differential, BF

Specimen Type

Body Fluid

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Body Fluid Ambient (preferred) 24 hours
  Refrigerated  24 hours

Clinical Information

Body fluids, other than the commonly analyzed urine and blood, include synovial, pleural, peritoneal, and pericardial fluids. These fluids may be present in increased volumes and may contain increased numbers of normal and abnormal cells in a variety of disease states.

Reference Values

TOTAL NUCLEATED CELLS

Synovial fluid: <150/mcL

Peritoneal/pleural/pericardial fluid: <500/mcL

NEUTROPHILS

Synovial Fluid: <25%

Peritoneal/pleural/pericardial fluid: <25%

LYMPHOCYTES

Synovial fluid: <75%

MONOCYTES/MACROPHAGES

Synovial fluid: <70%

Interpretation

Trauma and hemorrhage may result in increased red blood cells (RBC) and white blood cells (WBC); RBC predominate. WBC are increased in inflammatory and infectious processes:

-Neutrophils predominate in bacterial infections

-Lymphocytes predominate in viral infections

-Macrophages may be increased in inflammatory and infectious processes

-Eosinophils may be increased in parasitic or fungal infections

Clinical Reference

1. Kjeldsberg CR, Hussong, JW: Body Fluid Analysis. ASCP Press; 2015

2. Dyken PR, Shirley S, Trefz J, El Gammel T: Comparison of cyto-centrifugation and sedimentation techniques for CSF cyto-morphology. Acta Cytol. 1980 Mar-Apr;24(2):167-170

3. Sheth KV: Cerebrospinal and body fluid cell morphology through a hematologist's microscope, workshop presented at the ASCP-CAP Joint Spring Meeting, San Diego, March 1981

4. Schumacher AH, Reginato A: Atlas of Synovial Fluid Analysis and Crystal Identification. Lea and Febiger; 1991

Day(s) Performed

Monday through Sunday

Report Available

1 to 2 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

89051-Cell count with differential

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BFCC Cell Count and Differential, BF 34557-9

 

Result ID Test Result Name Result LOINC Value
SPEBF Fluid Type 14725-6
APPF Gross Appearance 9335-1
TNCF Total Nucleated Cells 55793-4
RBCF Erythrocytes 26455-6
CMTF Comment 48767-8
NEBFL Neutrophils 26513-2
LYBFL Lymphocytes 11031-2
MMBFL Monocytes/Macrophages 30437-8
EOBFL Eosinophils 26452-3
BABFL Basophils 28543-7
OTHBF Other Cells 75353-3
CMTBF Diff Comments 59466-3
OTHMR Other Cells Are: 75353-3
CMTBR Comment 48767-8
REVMR Reviewed by: 18771-6