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Test ID DCTR Direct Antiglobulin Test (Polyspecific), Blood

Shipping Instructions

Specimen must arrive within 48 hours of collection.

Specimen Required

Container/Tube: Pink top (EDTA)

Specimen Volume: 6 mL

Useful For

Demonstrating in vivo coating of red blood cells with IgG or the complement component C3d in the following settings:

-Autoimmune hemolytic anemia

-Hemolytic transfusion reactions

-Drug-induced hemolytic anemia

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
DATR Direct Antiglobulin Tst (Poly) No No
DC3TR Direct Antiglobulin Test (C3) No No
DIGTR Direct Antiglobulin Test (IgG) No No

Testing Algorithm

When the direct antiglobulin test (DAT) result is positive, the DAT test will be interpreted as “Invalid – See addl testing" and the polyspecific DAT test will be performed.


When polyspecific DAT result is positive, then DAT complement 3 and DAT IgG tests will be performed at an additional charge.

Method Name


Reporting Name

Direct Antiglobulin Test (Poly)

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

3 mL; Neonates: EDTA Micro tube 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient (preferred) 48 hours
  Refrigerated  48 hours

Reject Due To

Gross hemolysis OK

Clinical Information

IgG antibody or complement components secondary to the action of IgM antibody may be present on the patient's own red blood cells (RBC) or on transfused RBC.

Reference Values



Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.


Positive: Test polyspecific direct antiglobulin testing will be ordered and performed.


Autoimmune hemolytic anemia can occasionally occur with a negative direct antiglobulin test.


The direct antiglobulin test can be positive in some apparently healthy individuals.

Clinical Reference

Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017

Method Description

Antihuman globulin reacts with red blood cell-bound immunoglobulins to produce agglutination.(Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017)

Day(s) Performed

Monday through Friday, Sunday

Report Available

Same day/1 to 2 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DCTR Direct Antiglobulin Test (Poly) 1007-4


Result ID Test Result Name Result LOINC Value
DCTR Direct Antiglobulin Test (Poly) 1007-4