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Test ID ENAE Antibody to Extractable Nuclear Antigen Evaluation, Serum

Reporting Name

Ab to Extractable Nuclear Ag Eval,S

Useful For

Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive

 

Testing is not useful in patients without demonstrable antinuclear antibodies.

Profile Information

Test ID Reporting Name Available Separately Always Performed
SSA SS-A/Ro Ab, IgG, S Yes Yes
SSB SS-B/La Ab, IgG, S Yes Yes
SM Sm Ab, IgG, S Yes Yes
RNP RNP Ab, IgG, S Yes Yes
SCL70 Scl 70 Ab, IgG, S Yes Yes
JO1 Jo 1 Ab, IgG, S Yes Yes

Testing Algorithm

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

SS-A/Ro ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

SS-B/La ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

Sm ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

RNP ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

Scl 70 ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

Jo 1 ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 4 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86235 x 6

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ENAE Ab to Extractable Nuclear Ag Eval,S 90228-8

 

Result ID Test Result Name Result LOINC Value
JO1 Jo 1 Ab, IgG, S 33571-1
RNP RNP Ab, IgG, S 29958-6
SCL70 Scl 70 Ab, IgG, S 47322-3
SM Sm Ab, IgG, S 18323-6
SSA SS-A/Ro Ab, IgG, S 33610-7
SSB SS-B/La Ab, IgG, S 33613-1

Clinical Information

SSA, SSB, SM, and RNP are extractable nuclear antigens (ENA) that occur in patients with several different connective tissue diseases. Scl 70 (topoisomerase 1) is a 100-kD nuclear and nucleolar enzyme. Scl 70 antibodies are considered to be specific for scleroderma (systemic sclerosis) and are found in up to 60% of patients with this connective tissue disease. Scl 70 antibodies are more common in patients with extensive cutaneous involvement and interstitial pulmonary fibrosis, and are considered a poor prognostic sign.(1) JO1 is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells and a marker for the disease polymyositis.

 

For more information, see individual unit codes.

Interpretation

A positive result is consistent with a connective tissue disease. 

 

For more information, see individual unit codes.

Clinical Reference

1. Homburger H, Larsen S: Detection of specific antibodies. In Clinical Immunology: Principles and Practice. First edition. Edited by R Rich, T Fleisher, B Schwartz, et al. St. Louis, Mosby-Year Book, Inc, 1996, pp 2096-2109

2. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455

Analytic Time

Same day/1 day

Method Name

Multiplex Flow Immunoassay

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.