Sign in →

Test ID HDCH Cholesterol, High-Density Lipoprotein (HDL), Serum

Reporting Name

Cholesterol, HDL, S

Useful For

Cardiovascular risk assessment

Specimen Type


Specimen Required

Patient Preparation: Preferred but not required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tube must be centrifuged within 2 hours of draw time.

2. Red-top tube must be centrifuged and the serum aliquoted within 2 hours of draw time.

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  30 days

Reference Values

The National Lipid Association and the National Cholesterol Education Program (NCEP) have set the following guidelines for lipids (total cholesterol, triglycerides, high-density lipoprotein [HDL] cholesterol, low-density lipoprotein [LDL] cholesterol, and non-HDL cholesterol) in adults 18 years old and older:



≥40 mg/dL


≥50 mg/dL


The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for lipids (total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, and non-HDL cholesterol) in children 2-17 years old:


Low HDL: <40 mg/dL

Borderline low: 40-45 mg/dL

Acceptable: >45 mg/dL

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HDCH Cholesterol, HDL, S 2085-9


Result ID Test Result Name Result LOINC Value
HDCH Cholesterol, HDL, S 2085-9

Clinical Information

High-density lipoprotein (HDL) is the smallest of the lipoprotein particles and comprises a complex family of lipoprotein particles that exist in a constant state of dynamic flux as the particles interact with other HDL particles and with low-density lipoprotein particles and very-low-density lipoprotein particles. HDL has the largest proportion of protein relative to lipid compared to other lipoproteins (>50% protein).


Total cholesterol levels have long been known to be related to coronary heart disease (CHD). HDL cholesterol is also an important tool used to assess an individual's risk of developing CHD since a strong negative relationship between HDL cholesterol concentration and the incidence of CHD has been reported.


In some individuals, exercise increases the HDL cholesterol level; those with more physical activity have higher HDL cholesterol values.


Low high-density lipoprotein (HDL) cholesterol levels correlate with increased risk for coronary heart disease (CHD). Values greater than or equal to 80 to 100 mg/dL may indicate metabolic response to certain medications such as hormone replacement therapy, chronic liver disease, or some form of chronic intoxication, such as with alcohol, heavy metals, or industrial chemicals including pesticides.


HDL values of 5 mg/dL or less occur in Tangier disease, in association with cholestatic liver disease, and in association with diminished hepatocyte function.

Clinical Reference

1. Burtis CA, Ashwood ER, eds: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Elsevier Saunders; 2012

2. Rifai N, Warnick GR: Laboratory Measurement of Lipids, Lipoproteins, and Apolipoproteins. AACC Press; 1994

3. Jacobson TA, Ito MK, Maki KC, et al: National Lipid Association recommendations for patient-centered management of dyslipidemia: Part 1 - executive summary. J Clin Lipidol. 2014 Sep-Oct;8(5):473-488. doi: 10.1016/j.jacl.2014.07.007

4. Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents; National Heart, Lung, and Blood Institute. Expert panel on integrated guidelines for cardiovascular health and risk reduction in children and adolescents: summary report. Pediatrics. 2011;128;S213. doi: 10.1542/peds.2009-2107C

Report Available

1 day

Method Name

Enzymatic Colorimetric


If not ordering electronically, complete, print, and send a Cardiovascular Test Request (T724) with the specimen.