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Test ID HEMMF Hematologic Specified FISH, Varies

Ordering Guidance

Consult with the laboratory before ordering this test.


The fluorescence in situ hybridization (FISH) probes to be analyzed must be specified on the request when ordering, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not provided, this test may be canceled by the laboratory.


If testing paraffin-embedded tissue, bone marrow, or blood specimen for specific nonhematologic malignancies is desired, order MISCF / Miscellaneous Studies Using Chromosome-Specific Probes, FISH. If this test is ordered in this situation, it will be canceled and MISCF ordered and performed as the appropriate test.

Necessary Information

1. A list of probes requested for analysis is required.

2. A reason for testing should be submitted with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

3. A pathology and/or flow cytometry report may be requested by the laboratory to optimize testing and aid in interpretation of results.

Specimen Required

Submit only 1 of the following specimens:



Specimen Type: Bone marrow


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 2-3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.



Specimen Type: Whole Blood


Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

Useful For

The detection of specific chromosomal abnormalities in hematologic malignancies

Testing Algorithm

This test includes a charge for the probe application, analysis, and professional interpretation of results for one probe set (2 individual fluorescence in situ hybridization probes). Additional charges will be incurred for all additional probe sets performed.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

Hematologic Specified FISH

Specimen Type


Specimen Minimum Volume

Blood: 2 mL
Bone marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Clinical Information

Fluorescence in situ hybridization using gene-specific probes and various probe strategies can help characterize chromosome abnormalities in hematologic malignancies for diagnostic, prognostic, and therapeutic purposes.

Reference Values

An interpretive report will be provided.


A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.


The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.


This test is not approved by the US Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.


Bone marrow is the preferred specimen type for this fluorescence in situ hybridization test. If bone marrow is not available, a blood specimen may be used if there are malignant cells in the blood specimen (as verified by a hematopathologist).

Clinical Reference

Swerdlow SH, Campo E, Harris NL, et al, eds.: WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC Press; 2017

Method Description

This test is performed using commercially available and laboratory-developed probes. For enumeration and break-apart strategy probe sets, 100 interphase nuclei are scored; 200 interphase nuclei are scored when dual-color, dual-fusion fluorescence in situ hybridization probes are used. All results are expressed as the percent abnormal nuclei.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

9 to 12 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271 x 2, 88275, 88291-FISH Probe, Analysis, Interpretation; 1 probe set

88271 x 2, 88275-FISH Probe, Analysis; each additional probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HEMMF Hematologic Specified FISH In Process


Result ID Test Result Name Result LOINC Value
614267 Result Summary 50397-9
614268 Interpretation 69965-2
614269 Result Table 93356-4
614270 Result 62356-1
GC117 Reason for Referral 42349-1
GC118 Probes Requested 78040-3
GC119 Specimen 31208-2
614271 Source 31208-2
614272 Method 85069-3
614273 Additional Information 48767-8
614274 Disclaimer 62364-5
614275 Released By 18771-6

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HEMMB Probe, Each Additional (HEMMF) No, (Bill Only) No