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HelpTest ID IDENT Organism Referred for Identification, Aerobic Bacteria
Infectious
Reporting Name
Organism Refer for ID, Aerobic BactUseful For
Identification of pure isolates of aerobic bacteria
Differentiation of members of the Staphylococcus aureus complex (S aureus, Staphylococcus argenteus, Staphylococcus singaporensis, and Staphylococcus roterodami)
Specimen Type
VariesOrdering Guidance
Mayo Clinic Laboratories will not perform identification testing on suspected select agents (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis). Consult with your state health department or the Centers for Disease Control and Prevention regarding identification confirmation or exclusion of such isolates. For more information see www.selectagents.gov/sat/list.htm.
Additional Testing Requirements
If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibilities are not appropriate and not performed, ZMMLS will be canceled.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines for shipping information.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source (anatomical body site) is required.
2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.
Specimen Required
Preferred:
Specimen Type: Bacterial isolate swab
Supplies:
E-swab (T853)
Infectious Container, Large (T146)
Container/Tube:
Preferred: E-Swab collection and transport system
Acceptable: Flocked swab and 1-mL liquid Amies transport medium in 12 x 80 mm tube
Collection Instruction:
1. Perform isolation of infecting bacteria.
2. Utilize the flocked swab to obtain an adequate sample of pure cultured isolate. Do not submit mixed cultures.
3. Place swab into the transport system containing 1-mL liquid Amies transport medium.
4. If needed, break off end of swab and close the transport tube.
5. Place the transport system into the secondary infectious container for shipment.
6. Each isolate must be submitted under a separate order.
Note: For the following organisms, submit an agar slant or other appropriate media to ensure viability upon arrival to the laboratory; Neisseria gonorrhoeae, Campylobacter sp., Helicobacter pylori and any other fastidious organism.
Acceptable:
Specimen Type: Pure culture of organism from source cultured
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant or other appropriate media
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
3. Place the agar slant or other appropriate media into the secondary infectious container for shipment.
4. Each isolate must be submitted under a separate order.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reference Values
Identification of organism
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87077-Organism Referred for Identification, Aerobic Bacteria
87077-Identification Commercial Kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)
87147-Serologic Agglut Method 2 Ident (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87798-Identification by PCR (if appropriate)
Clinical Information
Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.
Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA gene.
Interpretation
Genus and species are reported on aerobic bacterial isolates, whenever possible.
Bacillus species will be reported as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.
Cautions
Isolates suspected of being select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) should be submitted to client's state health department or the Centers for Disease Control and Prevention for identification confirmation or exclusion.
Clinical Reference
1. Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019
2. Procop GW, Church DL, Hall GD, et al, eds. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott Williams and Wilkins; 2017
Method Description
Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, the utilized routine media include chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35° C in 5% carbon dioxide, the organism is identified using one or a combination of the following techniques: commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, and nucleic acid sequencing of the 16S ribosomal RNA gene.(JH Jorgensen, MA Pfaller, KC Carrol, et al. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015; Weyant RS, Moss CW, Weaver RE, et al. Identification of Unusual Pathogenic Gram-negative Aerobic and Facultatively Anaerobic Bacteria. Williams and Wilkins; 1996; Krieg NR, ed. Bergey's Manual of Systematic Bacteriology. Vol 1. Williams and Wilkins, 1984; Kolbert CP, Persing DH. Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol. 1999;2[3]:299-305)
Report Available
5 to 10 daysReject Due To
| Other | Agar plate |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.
NY State Approved
YesMethod Name
Dependent on organism submitted, 1 or more of the following methods will be used: Conventional Biochemical Testing, Commercial Identification Strips or Panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, and 16S RNA Gene Sequencing
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.