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Test ID LIVPR Liver Profile, Serum

Shipping Instructions

Ship specimen in amber vial to protect from light.

Necessary Information

Patient's age and sex are required.

Specimen Required

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Amber vial

Specimen Volume: 0.6 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged, and the serum aliquoted into an amber vial within 2 hours of collection.

Useful For

Screening for liver damage, especially if someone has a condition or is taking a drug that may affect the liver

Method Name

BILIT: Photometric, Diazonium Salt (DPD)

BILID: Photometric, Diazotized Sulfanilic Acid

AST: Photometric Rate, L-Aspartate with Pyridoxyl-5-Phosphate

ALT: Photometric Rate, L-Alanine with Pyridoxal-5-Phosphate

ALP: Photometric, p-Nitrophenol Phosphate

ALB: Photometric, Bromcresol Green

TP: Colorimetric, Biuret

Reporting Name

Hepatic Function Panel, S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated 7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Clinical Information

The hepatic function panel may be used to help diagnose liver disease if a person has signs and symptoms that indicate possible liver dysfunction. If a person has a known condition or liver disease, testing may be performed at intervals to monitor the health of the liver and to evaluate the effectiveness of any treatments. Abnormal tests on a liver panel may prompt a repeat analysis of one or more tests, or of the whole panel, to see if the elevations or decreases persist and may indicate the need for additional testing to determine the cause of the liver dysfunction.

Reference Values


0-6 days: Refer to for information on age-specific (postnatal hour of life) serum bilirubin values.

7-14 days: <15.0 mg/dL

15 days to 17 years: ≤1.0 mg/dL

>18 years: ≤1.2 mg/dL



≥12 months: 0.0-0.3 mg/dL

Reference values have not been established for patients who are under 12 months of age.




0-11 months: not established

1-13 years: 8-60 U/L

≥14 years: 8-48 U/L



0-11 months: not established

1-13 years: 8-50 U/L

≥14 years: 8-43 U/L




≥1 year: 7-55 U/L

Reference values have not been established for patients who are under 12 months of age.



≥1 year: 7-45 U/L

Reference values have not been established for patients who are under 12 months of age.




4 years: 149-369 U/L

5 years: 179-416 U/L

6 years: 179-417 U/L

7 years: 172-405 U/L

8 years: 169-401 U/L

9 years: 175-411 U/L

10 years: 191-435 U/L

11 years: 185-507 U/L

12 years: 185-562 U/L

13 years: 182-587 U/L

14 years: 166-571 U/L

15 years: 138-511 U/L

16 years: 102-417 U/L

17 years: 69-311 U/L

18 years: 52-222 U/L

≥19 years: 45-115 U/L



4 years: 169-372 U/L

5 years: 162-355 U/L

6 years: 169-370 U/L

7 years: 183-402 U/L

8 years: 199-440 U/L

9 years: 212-468 U/L

10 years: 215-476 U/L

11 years: 178-526 U/L

12 years: 133-485 U/L

13 years: 120-449 U/L

14 years: 153-362 U/L

15 years: 75-274 U/L

16 years: 61-264 U/L

17-23 years: 52-144 U/L

24-45 years: 37-98 U/L

46-50 years: 39-100 U/L

51-55 years: 41-108 U/L

56-60 years: 46-118 U/L

61-65 years: 50-130 U/L

≥66 years: 55-142 U/L

Reference values have not been established for patients who are under 4 years of age.



≥12 months: 3.5-5.0 g/dL

Reference values have not been established for patients who are under 12 months of age.



≥1 year: 6.3-7.9 g/dL

Reference values have not been established for patients who are under 12 months of age.


Hepatic function panel results are not diagnostic of a specific condition; they indicate that there may be a problem with the liver. In a person who does not have symptoms or identifiable risk factors, abnormal liver test results may indicate a temporary liver injury or reflect something that is happening elsewhere in the body-such as in the skeletal muscles, pancreas, or heart. It may also indicate early liver disease and the need for further testing and periodic monitoring.


Results of liver panels are usually evaluated together. Several sets of results from tests performed over a few days or weeks are often assessed together to determine if a pattern is present. Each person will have a unique set of test results that will typically change over time. A healthcare practitioner evaluates the combination of liver test results to gain clues about the underlying condition. Often, further testing is necessary to determine what is causing the liver damage or disease.


No significant cautionary statements

Clinical Reference

AACC: Lab Tests Online. Access 04/17/2018. Available at

Method Description

Bilirubin, Total:

Total bilirubin, in the presence of a suitable solubilizing agent, is coupled with 3,5-dichlorophenyl diazonium in a strongly acidic medium. The color intensity of the red azo dye formed is directly proportional to the total bilirubin and can be determined photometrically.(Package insert: Bilirubin Total Gen. 3, 07/2014. Roche Diagnostics, Indianapolis, IN)


Bilirubin, Direct:

Acidified sodium nitrite produces nitrous acid which reacts with sulfanilic acid (in acidic solution) to form a diazonium salt. The diazotized sulfanilic acid then reacts with bilirubin to form isomers of azobilirubin. In the direct bilirubin assay, only conjugated bilirubin is converted by the diazotized sulfanilic acid. The intensity of the color of azobilirubin is measured photometrically at 570 nm and is proportional to the direct (conjugated) bilirubin concentration.(Package insert: Roche Direct Bilirubin reagent; Indianapolis, IN, October 1999)


Aspartate Aminotransferase (AST):

AST is measured by a coupled enzyme kinetic method where the rate of decrease of NADH, determined at 340 nm, is directly proportional to the AST activity.(Package insert: Roche AST reagent, Indianapolis, IN, January 2000)


Alanine Aminotransferase (ALT):

ALT activity is determined by a kinetic method using a coupled enzyme reaction where the rate of NADH consumption is measured at 340 nm. The NADH decrease is directly proportional to the ALT activity.(Package insert: Roche ALT reagent, Indianapolis, IN, January 2000)


Alkaline Phosphatase:

In the presence of magnesium and zinc ions, p-nitrophenyl phosphate is cleaved by phosphatases into phosphate and p-nitrophenol. The p-nitrophenol released is directly proportional to the catalytic alkaline phosphatase activity. It is determined by measuring the increase in absorbance.(Package insert: Roche Alkaline Phosphatase reagent, Indianapolis, IN, February 2012)



The dye, bromcresol green (BCG), is added to serum in an acid buffer. The color intensity of the blue-green albumin-BCG complex is directly proportional to the albumin concentration and is determined photometrically.(Package insert: Roche Albumin reagent; Roche Diagnostic Corp., Indianapolis, IN, July 1999)


Protein, Total:

Divalent copper reacts in alkaline solution with protein peptide bonds to form the characteristic purple-colored biuret complex. Sodium potassium tartrate prevents the precipitation of copper hydroxide and potassium iodide prevents autoreduction of copper. The color intensity is directly proportional to the protein concentration which can be determined photometrically.(Package insert: Roche Protein reagent, Roche Diagnostic Corp., Indianapolis, IN 1999)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information








80076 (if appropriate if all analytes performed)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LIVPR Hepatic Function Panel, S 24325-3


Result ID Test Result Name Result LOINC Value
BILIT Bilirubin Total, S 1975-2
BILID Bilirubin, Direct 1968-7
AST Aspartate Aminotransferase (AST), S 30239-8
ALT Alanine Aminotransferase (ALT), S 1743-4
ALP Alkaline Phosphatase, S 6768-6
ALB Albumin, S 1751-7
TP Protein, Total, S 2885-2