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HelpTest ID MATCC Maternal Cell Contamination, Molecular Analysis, Varies
Useful For
Ruling out the presence of maternal cell contamination within a fetal specimen
Required for all prenatal testing performed in Mayo Clinic Laboratories' Molecular and Biochemical Genetics laboratories
Genetics Test Information
Required in conjunction with molecular and biochemical prenatal testing only.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CULFB | Fibroblast Culture for Genetic Test | Yes | No |
| CULAF | Amniotic Fluid Culture/Genetic Test | Yes | No |
| _STR1 | Comp Analysis using STR (Bill only) | No, (Bill only) | No |
| _STR2 | Add'l comp analysis w/STR (Bill Only) | No, (Bill only) | No |
Testing Algorithm
For prenatal specimens only: If amniotic fluid (nonconfluent cultured cells) is received, amniotic fluid culture/genetic test will be added and charged separately. If chorionic villus specimen (nonconfluent cultured cells) is received, fibroblast culture for genetic test will be added and charged separately.
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If this test is ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling or CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, and no other molecular testing is ordered, test will be changed to PPAP / Parental Sample Prep for Prenatal Microarray Testing, Blood.
Reporting Name
Maternal Cell Contamination, BSpecimen Type
VariesOrdering Guidance
If a prenatal specimen has already been submitted as part of another diagnostic test, a second prenatal specimen is not required. If a prenatal specimen has not yet been submitted, submit only 1 prenatal specimen.
Additional Testing Requirements
A maternal specimen and a prenatal specimen are both required.
-This test must be ordered on both the prenatal and maternal specimens under separate order numbers.
Shipping Instructions
Specimen preferred to arrive within 96 hours of collection.
Specimen Required
Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call 800-533-1710 for instructions for testing patients who have received a bone marrow transplant.
Specimen Type: Maternal blood
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Any anticoagulant
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Prenatal Specimens:
Submit only 1 of the following specimens:
Specimen Type: Cord blood
Container/Tube:
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Any anticoagulant
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Amniotic fluid
Container/Tube: Amniotic fluid container
Specimen Volume: 20 mL
Additional Information: A separate culture charge will be assessed under CULAF / Culture for Genetic Testing, Amniotic Fluid.
Specimen Stability Information: Refrigerated (preferred)/Ambient
Specimen Type: Cultured amniocytes
Container/Tube: T-25 flask
Specimen Volume: 2 full flasks
Collection Instructions: Submit confluent cultured cells from another laboratory.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Chorionic villi
Container/Tube: 15-mL tube containing 15 mL of transport media
Specimen Volume: 20 mg
Additional Information: A separate culture charge will be assessed under CULFB / Fibroblast Culture for Genetic Testing, Tissue.
Specimen Stability Information: Refrigerated
Specimen Type: Cultured chorionic villi
Container/Tube: T-25 flasks
Specimen Volume: 2 full flasks
Collection Instructions: Submit confluent cultured cells from another laboratory.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Minimum Volume
Amniotic Fluid: 10 mL
Blood, Cord Blood: 0.5 mL
Chorionic Villus: 5 mg
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
All specimens will be evaluated by Mayo Clinic Laboratories for test suitability.Clinical Information
One of the risks associated with prenatal testing is maternal cell contamination (MCC), which can occur when a fetal specimen comes into contact with maternal blood or tissue. The risk of MCC is associated with procedures such as chorionic villus sampling, amniocentesis, or extraction of fetal blood from the umbilical cord (cord blood). If MCC is present, the maternal DNA may mask the results of any genetic testing performed on the fetal DNA. Therefore, the results of prenatal testing may be compromised.
To rule out the presence of MCC, a maternal blood specimen is necessary for comparison of maternal and fetal chromosomal markers. The presence of both maternal and nonmaternal alleles for each fetal marker indicates the fetal specimen is not contaminated. MCC is confirmed when both alleles in the fetus are maternal.
Reference Values
An interpretative report will be provided.
Interpretation
An interpretive report will be provided.
Cautions
This test does not rule out the presence of low-level maternal cell contamination (<5%).
Method Description
The maternal blood genotype is compared to the fetal genotype, derived from amniocyte DNA or chorionic villus DNA, utilizing a PCR-based assay with a set of microsatellite repeat markers.(Unpublished Mayo method)
Day(s) Performed
Batched, performed most weekdays
Report Available
10 to 11 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81265
Fibroblast Culture for Genetic Test
88233-(if appropriate)
88240-(if appropriate)
Amniotic Fluid Culture/Genetic Test
88235-(if appropriate)
88240-(if appropriate)
Each additional specimen
81266
NY State Approved
YesForms
New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:
Clinical Reference
Nagan N, Faulkner NE, Curtis C, et al: Laboratory guidelines for detection, interpretation, and reporting of maternal cell contamination in prenatal analyses a report of the association for molecular pathology. J Mol Diagn. 2011 Jan;13(1):7-11, doi: 10.1016/j.jmoldx.2010.11.013
Method Name
Polymerase Chain Reaction (PCR) based comparison of Microsatellite Markers