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Test ID MHPRP Metamycoplasma hominis, Molecular Detection, PCR, Plasma

Useful For

Rapid, sensitive, and specific identification of Metamycoplasma hominis from plasma

 

This test is not intended for medicolegal use.

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

Mycoplasma hominis PCR, P

Specimen Type

Plasma EDTA


Shipping Instructions


Ship specimen refrigerated.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Metamycoplasma hominis DNA is unlikely.

 

Collection Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot

Submission Container/Tube: Screw-capped, sterile container

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and separate plasma within 24 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Clinical Information

Mycoplasma hominis has been associated with a number of clinically significant infections, although it is also part of the normal genital flora.

 

M hominis may be found in the respiratory specimens and spinal fluid of neonates. Although the clinical significance of such findings is often unclear, as spontaneous clinical recovery may occur without specific treatment, in premature infants, clinical manifestations of meningoencephalitis have been reported.

 

M hominis may play a role in some cases of pelvic inflammatory disease, usually in combination with other organisms. M hominis may be isolated from amniotic fluid of women with preterm labor, premature rupture of membranes, spontaneous term labor, or chorioamnionitis; there is evidence that it may be involved in postpartum fever or fever following abortion, usually as a complication of endometritis.

 

M hominis has rarely been associated with septic arthritis (including prosthetic joint infection), pyelonephritis, intraabdominal infection, wound infection, endocarditis, central nervous system infection (including meningoencephalitis, brain abscess, central nervous system shunt infection and subdural empyema), pneumonia, and infected pleural and pericardial effusions. Extragenital infection typically occurs in those with hypogammaglobulinemia or depressed cell-mediated immunity. In lung transplant recipients in particular, M hominis has been associated with pleuritis and mediastinitis. Recent evidence implicates donor transmission in some cases of M hominis infection in lung transplant recipients.

Reference Values

Not applicable

Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.

 

A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.

Clinical Reference

1. Cunningham SA, Mandrekar JN, Rosenblatt JE, Patel R: Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. Int J Bacteriol. Vol 2013, Article ID 168742. Available at http://dx.doi.org/10.1155/2013/168742

2. Sampath R, Patel R, Cunningham SA, et al: Cardiothoracic Transplant Recipient Mycoplasma hominis: An Uncommon Infection with Probable Donor Transmission, EBioMedicine 2017 May;19:84-90

3. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In Manual of Clinical Microbiology. 11th edition. Edited by JH Jorgensen. ASM Press, Washington, DC, 2015, pp 1088-1105

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MHPRP Mycoplasma hominis PCR, P 68546-1

 

Result ID Test Result Name Result LOINC Value
MPSRC Specimen Source 31208-2
44134 Mycoplasma hominis PCR, P 68546-1