Test ID MHPRP Metamycoplasma hominis, Molecular Detection, PCR, Plasma
Useful For
Rapid, sensitive, and specific identification of Metamycoplasma hominis from plasma
This test is not intended for medicolegal use.
Method Name
Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
Mycoplasma hominis PCR, PSpecimen Type
Plasma EDTAShipping Instructions
Ship specimen refrigerated.
Specimen Required
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Metamycoplasma hominis DNA is unlikely.
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Submission Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and separate plasma within 24 hours of collection.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Plasma EDTA | Refrigerated (preferred) | 7 days | |
Frozen | 7 days |
Clinical Information
Mycoplasma hominis has been associated with a number of clinically significant infections, although it is also part of the normal genital flora.
M hominis may be found in the respiratory specimens and spinal fluid of neonates. Although the clinical significance of such findings is often unclear, as spontaneous clinical recovery may occur without specific treatment, in premature infants, clinical manifestations of meningoencephalitis have been reported.
M hominis may play a role in some cases of pelvic inflammatory disease, usually in combination with other organisms. M hominis may be isolated from amniotic fluid of women with preterm labor, premature rupture of membranes, spontaneous term labor, or chorioamnionitis; there is evidence that it may be involved in postpartum fever or fever following abortion, usually as a complication of endometritis.
M hominis has rarely been associated with septic arthritis (including prosthetic joint infection), pyelonephritis, intraabdominal infection, wound infection, endocarditis, central nervous system infection (including meningoencephalitis, brain abscess, central nervous system shunt infection and subdural empyema), pneumonia, and infected pleural and pericardial effusions. Extragenital infection typically occurs in those with hypogammaglobulinemia or depressed cell-mediated immunity. In lung transplant recipients in particular, M hominis has been associated with pleuritis and mediastinitis. Recent evidence implicates donor transmission in some cases of M hominis infection in lung transplant recipients.
Reference Values
Not applicable
Interpretation
A positive PCR result for the presence of a specific sequence found within the Mycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.
A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.
Clinical Reference
1. Cunningham SA, Mandrekar JN, Rosenblatt JE, Patel R: Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. Int J Bacteriol. Vol 2013, Article ID 168742. Available at http://dx.doi.org/10.1155/2013/168742
2. Sampath R, Patel R, Cunningham SA, et al: Cardiothoracic Transplant Recipient Mycoplasma hominis: An Uncommon Infection with Probable Donor Transmission, EBioMedicine 2017 May;19:84-90
3. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In Manual of Clinical Microbiology. 11th edition. Edited by JH Jorgensen. ASM Press, Washington, DC, 2015, pp 1088-1105
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysTest Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
MHPRP | Mycoplasma hominis PCR, P | 68546-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
MPSRC | Specimen Source | 31208-2 |
44134 | Mycoplasma hominis PCR, P | 68546-1 |