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Test ID MYCO Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum

Advisory Information

Detection of IgM or IgG class antibodies to Mycoplasma pneumoniae provides exposure information. The preferred method of diagnosis of acute M pneumoniae infection is by molecular detection: MPRP / Mycoplasma pneumoniae, Molecular Detection, PCR.

Specimen Required

Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Useful For

Screen for recent or past exposure to Mycoplasma pneumoniae

Profile Information

Test ID Reporting Name Available Separately Always Performed
MYCOG M. pneumoniae Ab, IgG, S No Yes
MYCOM M. pneumoniae Ab, IgM, S No Yes
MYCON M. pneumoniae Ab Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MMYCO M. pneumoniae Ab, IgM, S by IFA No No

Testing Algorithm

If Mycoplasma pneumoniae antibodies, IgM is positive or equivocal, then M pneumoniae antibodies, IgM by IFA will be performed at an additional charge.

Method Name

MYCOG, MYCOM: Enzyme Immunoassay (EIA)

MMYCO: Indirect Immunofluorescence Assay (IFA)

Reporting Name

M. pneumoniae Ab, IgG and IgM, S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Clinical Information

Mycoplasma pneumoniae is an important respiratory tract pathogen. Several syndromes have been associated with the infection including pharyngitis, tracheobronchitis, pneumonia, and inflammation of the tympanic membrane presenting as bullous myringitis. 


M pneumoniae accounts for approximately 20% of all cases of pneumonia. Classically, it causes a disease that has been described as primary atypical pneumonia. The disease is of insidious onset with fever, headache, and malaise for 2 to 4 days before the onset of respiratory symptoms. Most cases do not require hospitalization. Symptomatic infections attributable to this organism most commonly occur in children and young adults (ages 2-19 years).

Reference Values

IgG: Negative

IgM: Negative

IgM by IFA: Negative


Positive IgM results are consistent with recent infection, although false-positives may occur (see Cautions).


A single positive IgG result only indicates previous immunologic exposure.


Negative results do not rule out the presence of acute or ongoing Mycoplasma pneumoniae-associated disease. The specimen may have been drawn before the appearance of detectable antibodies. If testing is performed too early following primary infection, IgG and IgM may not be detectable. If a Mycoplasma infection is clinically suspected, a second, convalescent specimen should be submitted in 14 to 21 days.

Clinical Reference

Smith T: Mycoplasma pneumoniae infections: diagnosis based on immunofluorescence titer of IgG and IgM antibodies. Mayo Clin Proc 1986;61:830-831

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Analytic Time

Same day/1 day

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86738 x 2-Mycoplasma pneumoniae by EIA

86738-Mycoplasma pneumoniae by indirect IFA (if appropriate)

LOINC Code Information

Result ID Test Result Name Result LOINC Value
MYCOG M. pneumoniae Ab, IgG, S 45224-3
MYCOM M. pneumoniae Ab, IgM, S 5257-1
MYCON M. pneumoniae Ab Interpretation 69048-7